Fig. 3.

Regulation of protein expression in trypanosomes. A, Comparison of the expression differences in the transcriptome and the proteome between PCF and BSF trypanosomes shows moderate correlation (Pearson coefficient 0. 57) for individual proteins and their transcript. B, Comparison of the ratios between PCF and BSF expression in transcriptome and proteome for gene ontology terms shows that the transcriptome is a good proxy for the regulation of many proteins like members of the glycolytic pathways in BSF and mitochondrial proteins in PCF. However, for some individual GO terms like oxidative stress response and citrate acid cycle differences between transcriptome and proteome are observable. C, Although the SILAC ratio for the protein subunits of the proteasome show an identical ratio between PCF and BSF, their transcripts show different ratios (blue: regulatory subcomplex, red: core subcomplex).