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. 2012 Oct 22;12(1):172–179. doi: 10.1074/mcp.M112.019224

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Fig. 5. — The putative DEAD-box RNA helicase is essential for viability in BSF. A, Alignment of core protein domains of homologs of the T. brucei RNA helicase (Tb09.211.4430) in L. major (LmjF.35.2270) and T. cruzi (Tc00.1047053510655.30). Alignments were performed with ClustalW2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/). B, Cumulative growth of parasites after induction of RNAi (red line), without induction (blue line) and of the parental strain (WT, black line). C, Images of WT parasites (top panel), un-induced RNAi cells (middle panel) and 96 h after induction (bottom panel). The surface of the cells was stained with sulfo-NHS-ATTO488 (green) and the DNA with DAPI (blue). The bright signal of the flagella pocket is marked with an arrow. Scale bars represent 6 μm.