Fig. 5.

Localization of PDLIM1 and filamin B in DEX-treated HTM cells. HTM cells treated with DEX for 6 days before being plated on fibronectin for 3 h were double-labeled with anti-PDLIM1 (A), anti-filamin B (D), rabbit IgG (G) antibodies and phalloidin (B, E, H). Merged images (C, F, I) are also shown. PDLIM1 colocalized with F-actin within CLANs and along stress fibers in both ethanol-treated (not shown) and DEX-treated cells, however, not all cells were positive for PDLIM1 (J). Although some CLANs did not contain filamin B, most CLANs ethanol contain filamin B regardless of the treatment. Insets show regions of cells with CLANs. Arrows point to five hubs or “vertisomes” of a CLAN that are interconnected by actin filaments; bar = 20 μm.