Table I. Changes in genes involved in assembly of the cytoskeleton. HTM1 and HTM2 are separate cell strains. The fold change (FC) for HTM1 is the average of 5 biological replicates. The FC for HTM2 is the average of 3 biological replicates. Abbreviations: ND, not detected; MS, mass spectrometry. * p < 0.05, † p < 0.01, ‡ p < 0.001.

GenBank	Name	Function	FC HTM1	FC HTM2	MS Protein Detected
NM_006403.3	NEDD9	Docking protein involved in tyrosine-kinase-based signaling related to cell adhesion, β1 integrin signaling	6.4‡	7.4‡	ND
NM_020992.2	PDLIM1	Binds α-actinin; recruits Clik1 kinase to F-actin	5.4‡	5.5‡	Yes
NM_000212.2	β3 Integrin	Binds ECM, signals to regulate cytoskeleton	4.8‡	6.7‡	Yes
NM_181523.2	PI-3 Kinase (regulatory subunit α)	Phosphorylates phosphatidylinositol; involved in integrin, GPCR, RTK and MAPK signaling	4.1‡	4.3‡	Catalytic subunit
NM_001753.4	Caveolin-1	Scaffolding protein involved in adhesion; regulates MAPK signaling	3.8†	3.8‡	Yes
NM_003603.5	SORBS2	Adaptor protein that promotes signaling complexes which regulates stress fibers	3.6†	4.2‡	SORBS3
NM_002652.2	Prolactin-induced protein	Belongs to the PIP family; interacts with AZGP1	3.3*	4.6‡	ND
NM_006449.3	CDC42 effector protein 3; Borg2	Regulation of F-actin & pseudopodia formation	3.1*	3.5‡	Yes
NM_001098424.1	Discs, large homolog 1; DLG1	Scaffolding protein for membrane recruitment of receptors, channels, signaling proteins	2.6*	3.3‡	Isoform 2
NM_001164317.1	Filamin B	Binds membrane proteins to actin cytoskeleton; may promote branching of actin filaments	2.5*	3.1‡	Yes
NM_001635.3	Amphiphysin	Control the association of membrane with cytoskeleton	2.5†	3.2‡	ND
NM_014945.2	ABLIM3	Mediates interaction between F-Actin & ABRA	2.5*	2.6‡	ABLIM1
NM_003633.2	ENC (with BTB-like domain)	Actin-binding protein involved in differentiation of neural crest cells	2.4‡	4.3‡	ND
NM_015150.1	Raftlin, lipid raft linker 1	May stabilize lipid rafts	2.3‡	2.4‡	Yes
NM_006393.2	Nebulette	Component of focal adhesion complexes	2.3‡	2.2*	LASP1
NM_001001522.1	Transgelin	Actin cross-linking/gelling protein	2.1*	1.9†	Yes
NM_001233.4	Caveolin-2	Scaffolding protein involved in adhesion; regulates MAPK signaling	2.1*	1.7†	ND
NM_006825.3	Cytoskeleton associated protein, CKAP	Stabilizes microtubules	−1.4*	−1.5†	Yes
NM_000638.3	Vitronectin	Extracellular matrix protein	−2.1†	−1.9†	ND
NM_014981.1	Myosin, heavy chain 15	Creates contractile force with F-actin	−2.7†	−2.1‡	ND
NM_001001548.2	CD36	Thrombospondin receptor	−10.2‡	−5.3†	ND