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. 2012 Oct 31;12(1):215–227. doi: 10.1074/mcp.M112.023176

Fig. 6.

Fig. 6.

Competition assays. Co-immunoprecipitation of T7-tagged FLNc d18–21 that was mixed with either 10 μm EEF-tagged Xin fragment (1686–1812) (A) or 10 μm myc-tagged Xirp2 fragment (2768–3327) (B) in the presence of increasing amounts (0 μm - 50 μm) of Xirp2 (A) or Xin (B), respectively. FLNc and coprecipitated Xin and Xirp2 fragments were detected using antibodies specific for their immunotags. Precipitated proteins and molecular mass standards are indicated by arrows and arrowheads, respectively. In most blots secondary antibodies also detected heavy and/or light chains of the T7-antibody. Note that increasing amounts of Xirp2 lessen but not completely impede binding of Xin to FLNc, and that Xin does not seem to reduce the efficiency of Xirp2 binding.