Fig. 2.

AGE and ubiquitin modification of PRPs. The protein lysate of control (CK) and diabetic (DK) rat kidney was separated on SDS-PAGE with and without tryptic digestion. Western blot analysis was performed with anti-AGE or anti-ubiquitin antibodies for detection of PRPs with AGE and ubiquitin modification. A, SDS-PAGE: Lane 2 and 3 showing undigested control (CK) and diabetic (DK) rat kidney lysate whereas lane 4 and 5 contains the same digested with trypsin (Try-CK, Try-DK). The same samples were immunoblotted with Anti-AGE (B) and Anti-ubiquitin (C) antibody, respectively. The experiment was repeated independently three times.