Fig. 3.

Total protease and proteasomal activity. Control (CK) and diabetic (DK) rat kidney protein lysate was assessed for total protease activity by A, Azocasein assay and B, In- gel protease assay. These assays demonstrated that total protease activity is significantly reduced in DK. Formation of fluorescent AMC from AMC-tagged peptide was determined to assess (C) Proteasomal activity of rat kidney homogenate of CK and DK, and D, In vitro MGO modified proteasome activity. Bars represent means ± S.E. from three independent experiments. *p < 0.05.