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Streptavidin Western blots of cathepsin labeling in RAW 264.7 cells. A, cells were treated with azido-E64 (20 μm, 0.5 h or 5 μm, 1 h). Lysates were prepared and labeled with DCG-04 to detect residual active cathepsins. B, reaction of cathepsins in situ; then lysates were made and detection took place using diol-reagent 3. C, click labeling of cathepsins in vitro using diol reagent 3.
