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. 2012 Nov 16;351(1):127–137. doi: 10.1007/s00441-012-1505-6

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© The Author(s) 2012

This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Fig. 1 — Clean hearts removed from female Anopheles albimanus mosquitoes injected with Saccharomyces cerevisiae and observed by light microscopy in fresh samples (a, b), in semi-thin sections stained with toluidine blue (c), and by transmission electron microscopy (d). Most of the yeast were aggregated and melanized (arrows) around the mosquito heart, but none were seen inside pericardial cells (PC). a Low magnification of a whole isolated heart showing pericardial cells (PC) and aggregated yeast (arrows) around dorsal vessel (dv). b Details of aggregated yeast (arrows) shown at higher magnification. c In semi-thin sections, yeast were seen close to PC (arrow) but were not phagocytozed (arrowhead one yeast cell lying between two PC). d Details of melanized yeast close to PC. Bars 200 μm (a, b), 50 μm (c), 5 μm (d)