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. 2012 Nov 16;288(1):181–191. doi: 10.1074/jbc.M112.382473

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FIGURE 1. — Co-localization of the GHS-R1a receptor with candidate GPCRs in Hek293A cells. Hek293a cells stably expressing the GHS-R1a receptor as an EGFP fusion protein were transduced with lentiviral vectors expressing candidate GPCRs as RFP fusion proteins. Co-localization of fluorescence was analyzed using confocal microscope and is indicated by yellow color overlap. A, co-localization (yellow) of the D₁ receptor (red) with the GHS-R1a receptor (green) was observed. B, co-localization of the MC₃ receptor (red) with the GHS-R1a (green) receptor was only observed intracellularly. C, co-localization of the 5-HT_2C receptor (red) with the GHS-R1a receptor (green) was demonstrated both intracellularly and on the membrane. D, the edited 5-HT_2C isoform, 5-HT_2C-VSV receptor (red), also demonstrated co-localization with the GHS-R1a receptor mainly in the intracellular space.