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Conversion of cobalt(II) factor III to cobalt(II)-precorrin-4 by UV-visible spectroscopy. The reaction was performed at 37 °C, with 5 μm cob(II)alt factor and 20 μm CbiH60 in the presence of 1 mm SAM and 10 mm DTT. Excess CbiH60 was added to ensure complete turnover. UV-visible scans were measured every 15 min. The inset shows the loss of absorbance at 595 nm (d bands) over time.
