Selective antagonists (L-732138 for NK1, SR48968 for NK2, and SB22200 for NK3 receptor) were used to identify the receptor subtype responsible for the observed U-251 cell migration.
A, effect of L-732138, SR48968, and SB22200 on the cell migration of U-251 induced by hHK-1. B, effect of L-732138, SR48968, and SB22200 on the cell migration of U-87 induced by hHK-1. The extraction of stained cells was measured at 600 nm. Using untreated cells as control, data were presented as the means ± S.E. for five independent experiments. ***, p < 0.001 versus hHK-1 controls.