FIGURE 7.

hHK-1-elicited up-regulation of MMP-2, and MT1-MMP was regulated by the phosphorylation of ERK, JNK, p38, and Akt. U-251 cells were pretreated with different kinase inhibitors for 30 min before stimulation with 1000 nm hHK-1. The following abbreviations are used: SP600125 (SP, 10 μm), SB203580 (SB, 10 μm), PD98059 (PD, 100 μm), and LY294002 (LY, 100 μm); control (ctl). 24 h later, the conditioned media were collected, and the cells were lysed. A, gelatin zymography analysis of conditioned medium from U-251 cells to detect the secretion of MMP-2. B, Western blotting analysis of whole cell lysates of U-251 MG to detect the protein level of MMP-2 and MT1-MMP. C, densitometric analysis of MMP-2 and MT1-MMP protein expression relative to GAPDH. Results are means ± S.E. for three independent experiments.