FIGURE 8.

Transcription factors AP-1 and NF-κB were involved in the induction of MMP-2 and MT1-MMP caused by hHK-1. U-251 cells were pretreated with different inhibitors for 30 min before stimulation with 1000 nm hHK-1 as follows: CAPE (25 μm), PDTC (100 μm), curcumin (Cur, 25 μm), and tanshinone IIA (T IIA, 25 μm). 24 h later, the conditioned media were collected, and the cells were lysed. A, gelatin zymography analysis of conditioned medium from U-251 cells to detect the secretion of MMP-2. B, Western blotting analysis of whole cell lysates of U-251 MG to detect the protein level of MMP-2 and MT1-MMP. C, densitometric analysis of MMP-2 and MT1-MMP protein expression relative to GAPDH. Results are means ± S.E. for three independent experiments.