TABLE 3.
Mass spectrometry analysis of proteins co-immunoprecipitating with CHERP
To identify proteins that may interact with CHERP, mass spectrometry data were filtered with two selection criteria: first, the assignment of ≥5 unique peptides and second, the ratio of peptide number assigned to the corresponding protein in CHERP over IgG >35. These criteria delimited 25 possible interactors shown in the table. Samples in bold indicate interactors predicted by database screening as highlighted in Fig. 6B.
| Protein name | Abbreviation | Accession No. | Molecular mass (kDa) | CHERP | IgG |
|---|---|---|---|---|---|
| Calcium homeostasis endoplasmic reticulum protein | CHERP | Q8IWX8 | 103 | 121 | 2 |
| U2 snRNP-associated SURP motif-containing protein | SR140 | O15042 | 118 | 115 | 3 |
| Splicing factor 3B subunit 2 | SF3B2 | Q13435 | 100 | 67 | 0 |
| Heterogeneous nuclear ribonucleoprotein U | HNRNPU | Q00839 | 91 | 52 | 0 |
| 116-kDa U5 small nuclear ribonucleoprotein component | EFTUD2 | B4DK30 | 105 | 46 | 0 |
| Bcl-2-associated transcription factor 1 | BCLAF1 | Q9NYF8 | 106 | 40 | 0 |
| Pinin | PNN | Q9H307 | 82 | 35 | 0 |
| Splicing factor 3B subunit 1 | SF3B1 | O75533 | 146 | 31 | 0 |
| Isoform 4 of Disks large homolog 1 | DLG1 | Q12959 | 100 | 18 | 0 |
| ATP-dependent RNA helicase DDX42 | DDX42 | Q86XP3 | 103 | 14 | 0 |
| Heterogeneous nuclear ribonucleoprotein U-like protein 1 | HNRNPUL1 | Q9BUJ2 | 96 | 13 | 0 |
| Protein 4.1 | EPB41 | P11171 | 97 | 13 | 0 |
| Isoform 3 of Importin-5 | IPO5 | O00410 | 117 | 13 | 0 |
| DNA-directed RNA polymerase | POLR1B | F5GZX4 | 132 | 12 | 0 |
| cDNA FLJ54400, highly similar to eukaryotic translation initiation factor 3 subunit 8 | NA | B4E1D5 | 104 | 12 | 0 |
| ATP-dependent RNA helicase A | DHX9 | Q08211 | 141 | 9 | 0 |
| Staphylococcal nuclease domain-containing protein 1 | SND1 | E7ESM6 | 101 | 9 | 0 |
| DIS3 | DIS3 | A8QI98 | 109 | 9 | 0 |
| DNA-directed RNA polymerase | POLR2B | C9J2Y9 | 133 | 8 | 0 |
| GC-rich sequence DNA-binding factor 1 | GCFC1 | Q9Y5B6 | 105 | 8 | 0 |
| Oxysterol-binding protein | DKFZp | Q68D75 | 97 | 7 | 0 |
| Rho GTPase-activating protein 11A | ARHGAP11A | Q6P4F7 | 114 | 7 | 0 |
| Protein flightless-1 homolog | FLII | Q13045 | 145 | 7 | 0 |
| Band 4.1-like protein 2 | EPB41L2 | O43491 | 113 | 7 | 0 |
| ATP-dependent DNA helicase Q5 | RECQL5 | B7Z8Y7 | 112 | 5 | 0 |
| Cell division cycle and apoptosis regulator protein 1 | CCAR1 | Q8IX12 | 131 | 5 | 0 |