FIGURE 7.

Bim silencing causes cell detachment and subsequent apoptosis. A, PPC1 cells were transfected with control or Bim siRNA, and after 48 h of transfection, floating and attached cells were collected. Whole cell lysates were subjected to Western blotting for caspase-3. LDH was used as loading control. B, PC3 cells were microinjected with multiple Bim antibodies or RbIgG antibody or Bim siRNA in the cytoplasm of cells. Cells were labeled live with DAPI, and floating cells were counted to monitor apoptosis after microinjection.