FIGURE 9.

Bim phosphorylation is accompanied by overexpression of Bcl-xL/Mcl-1, and Bim is sequestered by Mcl-1 and Bcl-xL. A, whole cell lysates prepared from normal prostate NHP9 cells, BPH1, PC3, LNCaP, PPC1 cells; breast normal human mammary epithelial cells (HMEC), MDA-MB231 (MDA-231), MDA-MB435 (MDA-435), MCF-7 cells; breast normal tissue (breast-N), and breast primary tumor (breast-PT) were subjected to Western blotting for indicated proteins. Actin serves as loading control. B, equal amounts of protein from LNCaP prostate cancer and NHP-9 normal prostate epithelial cells were immunoprecipitated with anti-Bim or Bcl-xL or Mcl-1 antibodies along with Rb- or Ms-IgG controls. Western blot analysis for indicated proteins was performed.