Effect of TSG-6 deficiency on HA in BAL and lung tissue. BAL and lung tissue samples were obtained from wild-type (white bars) and TSG-6−/− (black bars) mice. Mice were immunized with alum alone or with OVA/alum as indicated and subjected to OVA aerosol challenge. A, HA quantification in BAL using the HABP ELISA-like assay. B, HA quantification in lung tissue by the fluorophore-assisted carbohydrate electrophoresis assay normalized to dry weight. Data represent means ± S.E. for the amount of HA in the BAL (ng/ml) and the amount of HA in the lung (μg/g, dry weight (d.w)) (n ≥ 8). *, p < 0.05. KO, knock-out; I.P., intraperitoneal.