FIGURE 5.

Quantification of leukocytes bound to HA cables formed in the presence of TSG-6. MASM cells were either not treated (NT) or treated with only recombinant TSG-6, poly(I:C), or the simultaneous incubation of poly(I:C) + TSG-6 for 18 h. U937 monocytic cells were labeled with CM-DiI and applied to the MASM cells for 30 min at 4 °C, gently washing away unbound cells in 4 °C medium. Streptomyces hyaluronidase was added to the cultures in 4 °C medium for 5 min to release U937 cells bound to HA cables. The number of U937 cells remaining bound to the MASM cells with or without hyaluronidase treatment was measured as described under “Experimental Procedures”( n = 16). Error bars represent S.D.