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. 2012 Nov 19;288(1):442–454. doi: 10.1074/jbc.M112.413922

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FIGURE 3. — UNC93B1 augments surface localization of TLR3 in HEK293T cells. A and B, HEK293T cells were transfected with a TLR3-mCer (cyan) (A) or TLR9-YFP (yellow) (B) alone (top) or with UNC93B1 encoding plasmid (bottom). Plasma membrane markers SynaptoRed (A) and cholera toxin subunit B Alexa Fluor 555 (CTB-Alexa) (B) are shown in magenta. A and B, TLR membrane localization was evaluated from plots of normalized fluorescence intensities of TLR and plasma membrane (PM) within 3-μm line profiles (n = 9). Three representative lines are marked on merged images. Images are selected from five independent experiments. Scale bars, 10 μm.