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TLCS-induced calcineurin activation is Ca2+-dependent. A and B, both Ca2+ ionophores (A) A23187 (2 μm) and (B) ionomycin (1 μm) induced NFAT-luciferase activity. RLU, relative luciferase units; Ctl, control. C, pretreatment with BAPTA-AM prevented the increase. n = 3–4. *, #, p < 0.05, relative to the control or TLCS alone, respectively.
