FIGURE 5.

TLCS-induced acinar cell injury is dependent upon calcineurin activation. A, acinar cells were infected with Ad-Mcip1 24 h prior to TLCS (500 μm) administration. RFU, relative fluorescence units; Ctl, control. B–D, FK506 (10 μm) (B), CsA (1 μm) (C), or CiP (10 μm) (D) was administered at the stated time points relative to the start of a 2-h TLCS (500 μm) treatment. LDH leakage (left) and PI uptake (right) were measured to assess cell injury. n = 3–4. *, #, p < 0.05, relative to control and TLCS alone, respectively.