FIGURE 6.

The other naturally occurring bile acids cause acinar cell injury through a Ca²⁺ rise and calcineurin activation. A and B, pancreatic acinar cells were stimulated with TC (10 mm), TCDC (1 mm), taurodeoxycholic acid (TDC) (1 mm), or bile acid mixture (BAM) (0.5 mg/ml) for 2 h with or without BAPTA-AM (20 μm) (A) or the calcineurin inhibitors FK506 (10 μm), CsA (1 μm), or CiP (10 μm) (B). LDH leakage (left) and PI uptake (right) were measured to assess cell injury. n = 4. *, #, p < 0.05, relative to control and bile acid alone, respectively. RFU, relative fluorescence units; Ctl, control.