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CCTα is localized to the nucleoplasm and nuclear envelope in IEC-ras cells. IEC-18, ras3, ras4, and ras7 cells cultured on glass coverslips were immunostained with the following primary and secondary antibodies: rabbit anti-CCTα and AlexaFluor488-conjugated goat anti-rabbit; goat anti-lamin A/C and AlexaFluor594-conjugated donkey anti-goat. Images are 1-μm optical sections through the mid-nuclei captured using a Zeiss LSM520 Meta confocal microscope.
