FIGURE 4.

CCTα activity in cytosol and membranes of IEC-18 and IEC-ras cells. A, CCT activity was measured in the soluble (open bars) and membrane particulate fractions (black bars) from IEC-18 and IEC-ras in the presence or absence of PC/oleate vesicles as described under “Experimental Procedures.” Results are the mean and S.D. (error bars) of three experiments. B, the soluble and membrane particulate fraction (25 μg) of IEC-18 and IEC-ras cells were separated by SDS-8% PAGE and immunoblotted for CCTα. *, p < 0.05 compared with IEC-18 activity assayed under the same conditions.