FIGURE 7.

Activation and/or overexpression of CCTα does not grant anchorage-independent growth to IEC-18. After transfection with pCCTα-GFP or empty vector for 24 h, IEC-18 (500 cells) were cultured on 60-mm dishes coated with SeaPlaque agarose in media supplemented without or with 300 μm oleate complexed to BSA for 72 h. Cells were then seeded on plastic dishes, and colony formation was determined. CCTα-GFP overexpression relative to endogenous CCTα was determined after SDS-8% PAGE and immunoblotting using a CCTα antibody. Results are the means and S.D. (error bars) of three separate experiments.