FIGURE 6.

C1q alters fAβ association with neurons in an LRP1B-dependent manner. Mouse primary immature neurons were treated with 5 μm fAβ and/or 10 nm C1q for 24 h (A and B) or transfected with 10 nm scr siRNA or siRNA targeting LRP1B or GPR6 24 h before treatment with 5 μm fAβ ± 10 nm C1q (C), stained for MAP-2 (red), C1q (blue), and Aβ (green), and analyzed by confocal microscopy. A, images representative of three different experiments. Scale bar, 10 μm. B and C, scatter dot plot of Pearson's correlation coefficient for co-localization between Aβ and MAP-2 (n = 3, 5 fields per condition); red line, mean values. Results were compared using two-tailed non parametric Mann-Whitney U test (B) and one-way ANOVA (Kruskall-Wallis test) (C).