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. 2012 Nov 8;288(1):747–758. doi: 10.1074/jbc.M112.402529

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FIGURE 5. — S287A-STAT2 increases IFN-α induced tyrosine phosphorylation of STAT2 and STAT1 and is retained in the nucleus. U6A cells stably expressing WT-STAT2 or S287A-STAT2 were treated with IFN-α (1000 units/ml) for the indicated times. A, tyrosine phosphorylation of STAT2 (pSTAT2) and STAT1 (pSTAT1) were evaluated by immunoblot analysis. Quantification of A is depicted as bar graphs to the right. B, nuclear extracts were analyzed for the presence of activated STAT2 in the nucleus. GAPDH served as a negative control to check the purity of our extracts. Immunoblots shown are one representative experiment of three performed.