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Denaturant induced unfolding of W42R (red) and HGD (blue) by GdnHCl or urea (inset) at 37 °C. All samples contained 10 μg/ml protein in 100 mm sodium phosphate buffer, pH 7.0, 5 mm DTT, 1 mm EDTA and GdnHCl from 0 to 5.5 m and urea from 0 to 3 m. FI, fluorescence intensity ratio.
