FIG. 10.

Protective effect of α-TOCO on AMD/TNF-induced cytotoxicity.

Hepa1c1c7 cells were treated with 35µM AMD and/or 3ng/ml TNF, together with α-TOCO (100µM) or its vehicle. After incubation for the indicated time, ROS generation (A) and lipid peroxidation (B) were measured as described in Materials and Methods section. (C) TOCO was added to the medium at the concentrations indicated, and LDH release was measured after 48h. (D) TOCO (100µM) or vehicle was added to the medium, and caspase 3/7 activity was measured after 48h. Three-way ANOVA was applied. *Significantly different from the same treatment without TNF. ^#Significantly different from the same treatment without AMD. ^@Significantly different from the same treatment without antioxidant. p < 0.05, n = 3–5.