FIG. 11.

Effect of α-TOCO and pancaspase inhibition on AMD/TNF-induced cytotoxicity.

Cells were treated with 35µM AMD and/or 3ng/ml TNF, and TOCO (100µM) and/or z-VAD (40µM) were added to the incubation medium. After 48h, LDH release was measured as described in Materials and Methods section. Two-way ANOVA was applied. *Significantly different from the same treatment without TNF. ^#Significantly different from the same treatment without AMD. ^@Significantly different from the same treatment without TOCO. ^bSignificantly different from the same treatment without z-VAD. p < 0.05, n = 3–4.