Figure 3.

JNK signalling causes propagation of Yki activity to neighbouring cells. (A–D) Eye-antennal discs bearing GFP-labelled MARCM clones expressing Src64B (A–A′′′′′ and B–B′′′′′) and Src64B+Bsk^DN (C–C′′′′′ and D–D′′′′′) are shown. Caspase activity (A), JNK activity (B) or Yki activity (C and D) was visualized by anti-caspase-3 antibody staining (A), the puc-lacZ reporter (B), the diap1-lacZ reporter (C) or the ex-lacZ reporter (D). (A′′′′)–(D′′′′) and (A′′′′′)–(D′′′′′) are high-magnification images of the boxed area in (A′′)–(D′′). Cell nuclei were stained with DAPI (blue) (A′′′–D′′′). See supplementary Information online for genotypes. DAPI, 4,6-diamidino-2-phenylindole; GFP, green fluorescent protein.