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. 2012 Oct 27;4(10):274–283. doi: 10.4254/wjh.v4.i10.274

Table 1.

Antibody panel for flow cytometrric analysis of specific cells

Tubes Cell number Antibodies Dil. Target cells (tissue)
Control 1 x 105 No antibodies - -
1 1 x 106 CD45-PE-Cy7 (BD Biosciences) CD38-PE-Cy5 (eBioscience) 1:25 1:10 Hepatic stellate cells (liver)
2 1 x 106 CD45-PE-Cy7 (BD Biosciences) CD14-APC (eBioscience) CD34-PE (eBioscience) 1:25 1:10 1:10 Hematopoietic stem cells, fibrocytes and monocytes (BM, PB and liver)
3 1 x 106 CD45-PE-Cy7 (BD Biosciences) CD133-APC (eBioscience) CD34-PE (eBioscience) 1:25 1:10 1:10 Hematopoietic stem cells, fibrocytes and endothelial progenitor Cells (BM, PB and liver
4 1 x 106 CD45-PE-Cy7 (BD Biosciences) CD117-APC (eBioscience) CD34-PE (eBioscience) 1:25 1:10 1:10 Common progenitor cells (BM)

BM: Bone marrow; PB: Peripheral blood.