Figure 9.

Effect of p53 on the inflammatory response in models of endotoxin and chemical peritonitis. WT, pifithrin-α–treated WT, and p53^−/− mice are intraperitoneally injected with endotoxin. (A) Macrophages were collected and counted in peritoneal fluid 3 or 7 days after endotoxin injection. (B) F4/80⁺CD45⁺ macrophages collected 7 days after endotoxin injection were also studied by flow cytometry. Percentages indicated are M2 phenotype (CD206⁺). n=3 per group. P<0.05 for WT versus WT+pif or p53^−/−. (C) WT and p53^−/− mice are also treated with intraperitoneal thioglycolate and macrophages counted in peritoneal fluid 3 days after injection. (D) Harvested macrophages are stimulated with 100 ng/ml of endotoxin in vitro for 4 hours and the supernatant is collected for TNFα measurement. ^#P< 0.05. I/R, ischemia-reperfusion injury; pif, pifithrin-α.