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. 2012 Dec 26;24(1):43–52. doi: 10.1681/ASN.2012060571

Figure 1.

Figure 1.

Properdin differentially influences plasma C3 and C5 levels in CFH−/−.P−/− mice. (A) Plasma C3 levels in P−/−, CFH−/−, and CFH−/−.P−/− mice. Plasma C3 are measured by ELISA. Levels are normal in P−/− mice, whereas levels are dramatically reduced to a comparable extent in both CFH−/− and CFH−/−.P−/− animals. Horizontal bars denote median values. *P<0.001 versus either CFH−/− or CFH−/−.P−/− groups, Bonferroni multiple comparison test. (B) Western blot for C3 under reducing conditions using sera from P−/−, CFH−/−, and CFH−/−.P−/− mice. Intact C3 α-chain and β-chain are seen in P−/− mice. In contrast, absent and barely visible intact α-chain is seen in CFH−/− and CFH−/−.P−/− mice, respectively. In both strains, α-chain fragments are demonstrable. (C) Western blot for C5 under nonreducing conditions using sera from P−/−, CFH−/−, and CFH−/−.P−/− mice. Under these conditions, whereas plasma C5 is readily detectable in P−/− mice, it is absent in CFH−/− animals. C5 is readily detectable in CFH−/−.P−/− mice, although the intensity is less than that seen in P−/− animals. Lane one contains purified human C5. In both B and C, 1 μl of EDTA plasma from the examined strains is loaded on the gels.