Table II.

Impact of Trypsin Residue 39 on Cleavage of Inhibitors BPTI and APPI

Enzymes compared	Inhibitor	kcat (s−1) (S39)	kcat (s−1) (Y39)	Fold diff.
Cationic-Y39S/Cationic-wt	BPTI	6.3 ± 0.6 × 10−7	3.7 ± 0.4 × 10−7a	1.7
Mesotrypsin-wt/Mesotrypsin-S39Y	BPTI	1.9 ± 0.2 × 10−4a	3.1 ± 0.3 × 10−5	5.9
Cationic-Y39S/Cationic-wt	APPI	3.3 ± 1.1 × 10−5	1.8 ± 0.3 × 10−5b	1.9
Mesotrypsin-wt/Mesotrypsin-S39Y	APPI	4.2 ± 0.3 × 10−2b	2.4 ± 0.2 × 10−3	17.6

^aValues previously reported in Ref.10.

^bValues previously reported in Ref.11.