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(A) Quenching of N-MAC fluorescence by TcAChE. ○, 20 μM N-MAC; •, 20 μM N-MAC + 1.5 μM TcAChE; ▴, + 3 μM TcAChE; ▪, + 6 μM TcAChE; (B) Displacement of N-MAC from TcAChE by MB and by BW. •, 20 μM N-MAC; □, 20 μM N-MAC + 6 μM TcAChE; ▿, 20 μM N-MAC + 6 μM TcAChE + 0.200 μM BW; ▴, 20 μM N-MAC + 6 μM TcAChE + 20 μM MB.
