Expression of hMGL on K562 cell clones. One hundred thousand cells were incubated with MAb MLD-1 (anti-hMGL, ascitic fluid diluted at 1:100) on ice, washed, and incubated with fluorescein isothiocyanate-labeled F(ab′)2 of anti-mouse immunoglobulin G. The cells were analyzed with an Epics XL flow cytometer. These experiments were performed five times, and representative profiles are shown. CR4-4 and CR4-10 cells express hMGL at different levels, whereas control CR7 cells do not.