FIG. 2.
Increased infectivity by VSV pseudotyped with Ebola Zaire GP of K562 cells expressing hMGL. (A) K562 transfectants (105 cells per clone), including the CR7 control, were infected with VSVΔG* complemented with the wild-type Ebola Zaire GP, Zaire GP lacking the mucin-like domain (Zaire GPΔmuc), or VSV G. (B) Cells were incubated with anti-hMGL mouse MAb (MLD-1; 1:200 diluted ascites) or control antibody (1:200-diluted normal mouse ascites) for 30 min and infected with VSVΔG* complemented with Zaire GP or VSV G in the presence of the antibody. The infected cells were counted by fluorescence-activated cell sorting, and background infectivity was subtracted from each data set. The relative percentages of infectivity were determined by setting the number of infected CR7 cells to 100. All experiments were done three times, and the means and standard deviations are shown.