FIG. 2.
(A) Structural organization of the murine HIPK2 gene. The bioinformatic analysis is described in Materials and Methods. Exons are represented by filled boxes. Potential alternative splicing occurring between exons 16 and 19 according to the numbering of model maker software (http://www.ncbi.nlm.nih.gov/mapview) are labeled 2a and 2b. (B) Analysis of the HIPK2 gene expression in different murine tissues by RT-PCR. Total mRNA (Clontech) was reverse transcribed, and the HIPK2a and HIPK2b cDNA and 18S rRNA were amplified with primers described in Materials and Methods. Positions of the DNA fragments corresponding to 18S, HIPK2b, and HIPK2a are indicated to the left of the figure. Sizes of the DNA fragments are indicated to the right of the figure in base pairs.