FIG. 1.

Proteasome-modulating agents augment rAAV transduction in airway cell lines. The effects of proteasome-modulating agents LLnL, aclarubicin (ACL), and doxorubicin (DOX) on AV2.Luc and AV2/5.Luc transduction of immortalized human airway cell lines IB3 (A) and A549 (B) were evaluated. Proteasome-modulating agents were coadministered with each rAAV vector (MOI of 500 particles/cell) at the time of infection, and transduction was evaluated 24 h later. Various concentrations of each chemical were evaluated, as indicated on each graph. Luciferase activity was measured with a luminometer at 47.7% sensitivity. Data represent the means (± standard errors of the means) of the relative luciferase activity (per well) results from four independent experiments.