FIG. 2.

Proteasome-modulating agents augment rAAV transduction from the apical membrane of polarized human airway epithelia. Primary bronchial human airway epithelia were cultured and differentiated at an air-liquid interface on transwell filters 1 cm in diameter. Particles (10⁹) of AV2.Luc or AV2/5.Luc in a volume of 50 μl were applied to the apical surface of airway epithelia in the presence or absence of LLnL (40 μM), Z-LLL (4 μM), aclarubicin (ACL; 0.25 μM), or doxorubicin (DOX; 5 μM). Luciferase activity was measured at 3 days (A) and 17 days (B) postinfection with a luminometer setting of 80% sensitivity. Data represent the means (± standard errors of the means) of the relative luciferase activity (per well) results from three independent experiments.