FIG. 3.

Matrix dose-response assays of rAAV-2 transduction with combined application of LLnL and doxorubicin. IB3 (A) and A549 (B) cells, cultured in 96-well plates, were infected with 500 particles/cell of AV2.Luc. Different concentrations of each proteasome inhibitor alone or in combination (as indicated on each axis) were applied at the time of infection. At 24 h postinfection, luciferase activity was measured with a luminometer at 47.7% sensitivity with 1/10 of the total cell lysate. The level of luciferase expression in the diagrams represents the total from each well. The color signifies the range of the mean relative luciferase activity (measured in relative luciferase units [RLU]) per well for four independent experiments.