TABLE 1.
Growth conditions and characteristics of sE trimer crystals
| Growth conditions (precipitant, pH) | Space groupa | No. of molecules per asymmetric unit | Resolution (Å) |
|---|---|---|---|
| Ammonium sulfate, 7.8 | P4222 | 3 | 3.5b |
| Ammonium sulfate, 7.8c | P213 | 2d | 3.5 |
| PEG, 4.5e | P21212 | 3 | 3.2 |
| PEG, 4.5e | C222 | 3 | 3.2 |
| PEG, 4.5e | P212121 | 6 | 2.7 |
a
As determined from analysis of a dataset (92 to 100% complete) and extending to at least 3.5 Å.
b
Due to strong anisotropy, the diffraction pattern extends to 3 Å in one direction but not beyond 3.8 Å in the orthogonal plane, yielding an overall approximate resolution limit of 3.5 Å.
c
Crystals appeared in the same conditions (sometimes in the same drops) as those of the tetragonal form.
d
For this crystal form, the threefold axis of the trimer coincides with a crystallographic axis.
e
The three orthorhombic crystal forms appeared in similar conditions with various PEGs (2000 to 20000) and could only be distinguished by X-ray diffraction analysis.