FIG. 5.

Antibody-mediated inhibition of fusion. HeLa cells were infected with VV-T7 and then transfected with gH-Y835A+gL (A and B) or gH-S830stop+gL (C). At 4 h posttransfection, 40 μl of MAb 206 was added for overnight incubation at 37°C (B and C only). At 18 h posttransfection, samples were processed for the steady-state confocal assay with MAb 258 and goat anti-mouse Alexa 488 to detect gH+gL-transfected cells. The bar in panel A is 25 μm.