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Details of Multiplex PCR primer implemented in the satudy
| Primer pairs | Location in DNA | Sequence |
|---|---|---|
| I | ESV | 5’-GTT.CCA.TGT.GAA.CAG.CAG.T-3’ |
| 5’-CGA.AAA.CAT.ACG.ACA.ACT.GC-3’ | ||
| II | ITS1 | 5’-GCT.ACA.TCC.TTT.TGA.TCT.GTT-3’ |
| 5’-AGA.CAC.AAT.ATC.AAC.CAC.AGT.ACA-3’ | ||
| III | ITS1 | 5’-GCG.GAA.GGA.TCA.TTA.TCG.TGT.A-3’ |
| 5’-TGG.ATT.ACA.AAG.AAA.ACC.ATC.ACT-3’ | ||
| IV | ITS2 | 5’-GTG.AGC.GTA.ATA.AAG.GTG.CAG-3’ |
| 5’-TTC.ATC.ACA.CAT.CTT.CCA.CTA-3’ | ||
| V | ITS2 | 5’-CAA.TTG.AAA.ACC.GCT.TAG.CGT.GTT.T-3’ |
| 5’-TGA.TCT.GAG.GTC.GAC.ATT.TCC-3’ |
