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Plasma hF.IX levels after intraportal administration of 1011 VG of AAV-hF.IX16 pseudotypes in C57BL/6 mice. The AAV pseudotypes contained an hF.IX expression cassette flanked by AAV2 ITRs packaged inside an AAV2 capsid (AAV2/2), an AAV6 capsid (AAV2/6), or an AAV8 capsid (AAV2/8). Vectors were purified by cesium chloride gradient centrifugation (CsCl) or heparin sulfate column chromatography (HPLC).
