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. 2004 Mar;78(6):3110–3122. doi: 10.1128/JVI.78.6.3110-3122.2004

FIG. 10.

FIG. 10.

Real-time PCR quantitation of sc and ss AAV.luc genomes in genomic DNA extracted from the livers of BALB/c mice 1 year after vector injection. (A) sc or ss AAV.luc VG were quantified using three sets of primer-probe combinations. ss VG were undetectable in mice that had been injected with 5 × 109 or 5 × 1010 VG. (B) In contrast, sc VG were reproducibly detected with all three primer-probe sets, even though only the low dose of 5 × 109 VG had been injected. (C) Luciferase expression in three representative mice 1 year after vector injection. (i) Mouse injected with 5 × 109 VG of ss AAV.luv; (ii) mouse injected with 5 × 1010 VG of ss AAV.luc; (iii) mouse injected with 5 × 109 VG of SC AAV.luc. One of the mice in the group injected with 5 × 109 VG of the ss vector died before the 1-year time point.