FIG. 9.

A time course of expression from sc versus ss AAV2/2 vectors expressing luciferase. BALB/c mice (n = 2 per virus) were injected with 5 × 10⁹ VG of sc AAV2/2.luc (▪), 5 × 10¹⁰ VG of ss AAV2/2.luc (▴), or 5 × 10⁹ VG of ss AAV2/2.luc (•). (A) Mean luciferase expression for all three virus groups plotted on a log scale. Luciferase expression from the sc vector was up to 50-fold higher than luciferase expression from the same dose of ss vector and remained high over the 1-year period of study. (B to D) Luciferase expression from sc vectors as well as ss vectors gradually increased over the first 5 weeks after vector injection. The expression profiles for two individual animals per virus dose are shown.