Fig. 1.

Immunogenicity of B8, C2 and C1 peptides in HHDII mice

Four peptides of Leishmania gp63 proteins were predicted for HLA-A2.1 using SYFPEITHI web-based software. 100µg of each peptide was injected S.C. at the base of tail of HHDII mice together with the helper peptide and IFA adjuvant. A week after the immunization, spleens were harvested and splenocytes were cultured with spleen blast cells pulsed with relevant and irrelevant P53 peptides for 5 days. On day 5 the cells were used as effectors against target cells “RMAS-A2” pulsed with relevant and irrelevant P53 peptides using standard 4-hour cytotoxicity assay. Results of peptides B8, CM4 & C2 are representative of immunogenic peptides while peptide C1 represents a poor immunogenic peptide. Graphs represent a number of independent positive experiments as Table 3